Pharmaceutical Guidelines

High Performance Liquid Chromatography (HPLC) HPLC is used in pharmaceuticals for analysis of dosage forms before send these to market because HPLC is most accurate method for analysis. High performance liquid chromatography (HPLC) is a fast column liquid chromatography method where a solvent is passed through a column under high pressure (of up to 400atms). Like every other form of chromatography, HPLC separates a liquid sample into its constituent parts on the basis of the differences between molecules of the subject mixture and the molecules mobile and stationary phases. For chromatography to occur, there must be a stationary phase and a mobile phase. The adsorbent normally is a solid (or a liquid) in nature and the mobile-phase normally a liquid or a gaseous matter. The solvent is responsible for carrying the constituents of the subject mixture through the stationary phase. More lagging is experienced in components which interact more with the stationary phase. In ...

Guideline for Preparation of Mobile Phase Required for HPLC   Learn guidelines for preparation and validation of the mobile phase for HPLC analysis. Mobile Phase Preparation 1. Use HPLC grade/ AR Grade / GR grade chemicals. 2. Weigh / Measure each component of the mobile phase separately. 3. Degas each solvent and buffer by sonication and then filter each component of the mobile phase separately through 0.45μ filter (Use the filterpaper as per suitability with solvent). 4. Mix the individual solvent and buffer as per the specified proportion. 5. Adjust the pH of the mobile phase wherever specified (i.e. before or after mixing of individual components) between ± 0.05 unless otherwise specified. 6. Sonicate the mobile phase for 10 minutes or more as required. 7. Ensure that mobile phase is free of particles. Do not use the mobile phase if particles are observed. 8. Enter the details of mobile phase preparation in laboratory note book. Related: Theoretical Pl...

Difference between GC and HPLC Techniques Gas Chromatography and High Performance Liquid Chromatography both are used to detect the components of the compounds but both are used for different purposes because both have differences in their working and use. Gas Chromatography and the High Performance Liquid Chromatography system are separation techniques used in obtaining specific eluents from an analyte basing on the compounds in the analyte distribution between the two phases (Mobile phase and Stationary phase). The mobile phase is the part in chromatography which moves and it usually contains the sample while the stationary phase is one which does not move and contains adsorption material. Chromatography is a larger analytical technique in chemistry. In modern-day analytical chemistry, Gas Chromatography and the High Performance Liquid Chromatography system are the major techniques used in Analytical Chemistry. In an earlier analysis technique such as the Ion Exchange Chr...

Differences between HPLC and UPLC HPLC and UPLC both are used for analysis of the pharmaceutical compounds but both have some differences. These differences are discussed in this article.   Liquid chromatography is a technique that is utilized to separate any sample into its individual components. In this case, the chromatographic procedure involves a liquid as the moving phase. HPLC and UPLC stand for High Performance Liquid Chromatography and Ultra Performance Liquid Chromatography respectively. It is understandable to find these terminologies quite daunting, but at its core HPLC and UPLC are both used to separate the various components of compounds found in mixtures, using high pressures to push solvents through columns. However, the similarities between the two end there and the differences between HPLC and UPLC begin. UPLC operates at considerably high pressures of 15,000 psi (psi, in this case, stands for Pounds per square inch and is a unit of pressure), whil...

10-TIPS-FOR-HPLC-ANALYSIS. Tips for HPLC Analysis In Pharmaceuticals HPLC analysis is critical and have a lot of chances of errors after a safe handling. These are some tips those'll help you in a error free analysis. HPLC, also known as High-Performance Liquid Chromatography system , is one of the most crucial analytical methods in pharmaceuticals. The purpose of HPLC is the separation of each compound that make up a mixture. It passes pressurized liquid solvent with the use of pumps which holds the sample mixture via a column full of solid adsorbent substance. There is a difference in the flow rates for each component due to the interaction of the components with the absorbent which causes the components to separate as they flow out. HPLC Used in the Pharmaceutical Industry This analysis methodology is used to test and detect raw elements or components used in making pharmaceutical products with the use of qualitative and quantitative analytical technique. I...

Basics   of   Cleaning   Validation Cleaning method validation has its great importance in pharmaceutical manufacturing. An improper cleaning can produce harmful product. This article provides a brief introduction of whole process of cleaning validation. Cleaning of pharmaceutical instruments after a product has been manufactured is critical to ensure that the subsequent products to be manufactured are not contaminated. The removal of the residues of the previous products from manufacturing equipment is known as cleaning. If the whole process of cleaning has been documented, it is referred to as cleaning method validation. Cleaning validation is proof that the cleaning process is effective to removed all residues of the product that was manufactured, cleaning agents those were used during the cleaning process and prevents micro-organisms from developing. This process is done as a requirement of regulatory authorities. Manufacturing companies should not d...

C18   HPLC   Columns   and   Their   Properties HPLC columns separate any compound into its components and C18 columns are widely used in chemical analysis because these have some unique properties. Standards and success of any pharmaceutical company is determined by how professional chemical analysis   are being under taken. Chromatography remains the most important technique for identifying and analyzing a wide variety of chemical samples within pharmaceutical industries. The technique does not only help chemical analysis in scientifically analyzing compositions of various drugs during their development but also key in identifying compounds that may be unknown. Chromatography, therefore, apart from helping in chemical identification, also ensures purity of chemical mixtures. Selection of the most suitable chromatography methods is of paramount importance for an effective, and efficient samples analysis. There are various types of separation system...

Difference between C8 and C18 Columns Used in HPLC System C8 and C18 both types of columns are used in pharmaceutical analysis but their use are specific for analysis of different products. High Performance Liquid Chromatography system (HPLC) refers to a technique in analytical chemistry that is used to identify, separate and quantify each component in a mixture. C8 and C18 both refer to the alkyl chain of a bonded face of a column. They are both used in the high performance liquid chromatography system. The two columns have some similarities, but they are also different in some way. Some of the differences between C18 and C8 are discussed here. Octadecylsilane (C18) has 18 carbon atoms. On the other hand, Octylsilane (C8) has only 8 carbon atoms on the column parking bonded to silica(Si). C18 will tend to retain more than C8. In that, if a similar compound was eluted on the two columns, it will elute faster on C8 and slower on C18.This is caused by the parking per unit su...

Name Of Post :   Recruitment For Pharmaceutical Industry Post Date:                24/07/2020 Post Update Date:    24/10/2020 Short Information: Serves patients by preparing medications, giving pharmacological information to the multidisciplinary health care team, and monitoring patient drug therapies. Prepares medications by reviewing and interpreting physician orders and detecting therapeutic incompatibilities. Dispenses medications by compounding, packaging, and labeling pharmaceuticals. Controls medications by monitoring drug therapies; advising interventions. Completes pharmacy operational requirements by organizing and directing technicians’ workflow, verifying their preparation and labeling of pharmaceuticals, and verifying order entries, charges, and inspections. Provides pharmacological information by answering questions and re...